Part:BBa_K1379051:Design
S. pneumoniae ComE CDS
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Removal of SpeI illegal site
Source
iGEM 2014 Hong_Kong_HKUST Team has cloned comE from S. pneumoniae strain NCTC7465 by PCR, followed by Gibson Assembly into pSB1C3 backbone.
References
B. Martin et al "ComE/ComE~P interplay dictates activation or extinction status of pneumococcal X-state (competence) " 2012
B. Martin et al "Cross-regulation of competence pheromone production and export in the early control of transformation in Streptococcus pneumoniae " 2000
B. Martin et al "Expression and maintenance of ComD–ComE, the two-component signal-transduction system that controls competence of Streptococcus pneumoniae mmi_7071 1513..1528 "
Cheng, Q., Campbell, E.A., Naughton, A.M., Johnson, S., and Masure, H.R. (1997) The com locus controls genetic transformation in Streptococcus pneumoniae. Mol Micro-biol 23: 683±692.
Pestova, E.V., and Morrison, D.A. (1998) Isolation and characterization of three Streptococcus pneumoniae transformation-specific loci by use of a lacZ reporter insertion vector. J Bacteriol 180: 2701±2710.
Ween, O., Gaustad, P., and Havarstein, L.S. (1999) Identification of DNA binding sites for ComE, a key regulator of natural competence in Streptococcus pneumoniae. Mol Microbiol 33: 817±827.